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The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 °C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 μM FOXO4-DRI was added in the medium immediately

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However, BPC-157's angiogenic properties could theoretically affect the pharmacokinetics of injected biologics by altering tissue blood flow at injection sites