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Laser tattoo removal operates on the principle of selective photothermolysis: delivering laser energy at a wavelength that the target pigment absorbs preferentially, in a pulse short enough to shatter the pigment particles without damaging surrounding tissue

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After extensive washing with 1% (v/v) acetic acid, the protein-bound dye was dissolved in 10 mM Tris base solution and the optical density was determined at 515 nm